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The measurement of vitamin D using blood spot card samples is a reliable means of collecting and measuring vitamin D levels.
Blood card samples (100 µL whole blood) were extracted for DA using a water (60%)/methanol (40%) solution (2.0 ml) according to the methods of Maucher and Ramsdell [55].
In addition, eight FTA card samples and three DCM samples did not provide valid results for the Cobas® 4800 test.
The AdV fiber gene (a polymorphic gene that can be used to distinguish AdV species) was screened in Guthrie card samples by using semi-nested PCR.
Adenovirus DNA is detected in Guthrie card samples at too low a frequency to reveal an association between adenovirus and the development of leukaemia.
Whole genome amplification (WGA) from 1.2 mm diameter trephine punched discs were used to increase and preserve the finite amount of DNA available from the FTA card samples.
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The cards were placed in the Micro Fluidic Card Sample Block of an ABI Prism 7900 HT Sequence Detection System Applied Biosystemss).
The socioeconomic disparities in birthweight (Table S2) were closer to the results from the recall sample but not the card sample.
The social patterning of birthweight among children whose mothers reported their birthweight differed from that among children in the health card sample (Table 3).
A 100 gram difference in a child's birthweight in the card sample was associated with decreased likelihood of stunting (RR: 0.97; 95% CI: 0.96, 0.98), underweight (RR: 0.95; 95% CI: 0.94, 0.96), and wasting (RR: 0.96; 95% CI: 0.94, 0.97) at an older age in the card sample.
The difference between the card sample and the maternal recall sample in the distribution across household wealth, parental education, caste, religion, gender, and urban residence was compared using multilevel models.
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