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We have previously published a CarD alignment (Srivastava et. al, 2013) based on 452 sequences that represented the six diverse groups of bacteria in which CarD is found.
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3) To increase clarity of the overall presentation, the authors should provide an additional (supplemental) figure showing a protein sequence alignment of CarD from different organisms, to support the claim that CarD residue W86 is universally conserved.
(A ) Sequence alignment of the CARD domain of MAVS from different species.
A larger alignment of 831 CarD sequences is included (Source code 1 ).
We also include a large data supplemental file (Supplementary data file 1) containing an alignment of 831 CarD sequences.
(A ) Sequence alignment of the MAVS CARD from 10 different species with the secondary structures based on the X-ray model of human MAVS CARD.
SW# is slower only for very long alignments on single GPU card.
An amino acid alignment of the prodomain of CARD containing caspases, including B. mori caspase-5, shows a low amino acid conservation, but a high conservation of the position of the 6 alpha helices (additional file 10).
The positive impact of an educational card game found in this research is in alignment with previous studies (Da Rosa et al., 2003; Eckert et al., 2004; Beylefeld and Struwig, 2007; Valente et al., 2009).
The new division alignment is intriguing because five wild-card teams will join the division winners in the playoffs.
Auditory-visual stimuli were aligned to the millisecond using the audio card and a photo-detector connected to an oscilloscope for auditory-visual output signals alignments.
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