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First, microcapsules were washed with 200 mM sodium carbonate buffer pH 10 by centrifugation.
High-binding 96-well ELISA plates (Greiner) were coated with 30 ng 4G2 antibody (clone D1-4G2-4-15) in carbonate buffer, pH 9.6 overnight at 4 °C.
Some experiments with carbonate buffer were also repeated in a wetted wall column.
Experiments were performed in a channel absorber using CO2 absorption into carbonate buffer solutions containing arsenite as a catalyst.
Alkalinity, total inorganic carbonate buffer.
Volatile organic acids/total inorganic carbonate buffer.
As control, another immunotube was incubated with carbonate buffer alone.
The peptide was dissolved in carbonate buffer to 10 µg/mL for plate coating.
The core RNAP was then dialyzed into carbonate buffer and concentrated for labeling as described below.
Recombinant HtpG proteins were dissolved in carbonate buffer to 1 µg/mL for plate coating.
RX was dissolved in DMSO and diluted in 0.5 M carbonate buffer, pH.
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