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The availability of carbon, nitrogen source, and inducer concentration are the crucial factors affecting the enzyme production (Majeau et al. 2010).
Eight factors viz., carbon, nitrogen source (urea), 2,5-xylidine (inducer), wheat bran (ligninocellulosic material), inoculum size, fermentation broth pH, yeast extract and phosphate source (KH2PO4) at three levels with a OA layout of L18 (21 × 37) were selected for the proposed experimental design.
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However, this isolate of yeast was separately optimized for higher decolorization at different medium with varying contents of carbon, nitrogen sources and their different concentrations.
A bacterial species was originally defined using a combination of morphology and simple biochemical tests such as the utilization of specific carbon, nitrogen sources, and their reaction to the Gram stain.
Some of the carbon-nitrogen sources and the environmental factors affecting the production of biosurfactants selected from oil industry are discussed here.
Only experiments from the "carbon source", "nitrogen source", "carbon and nitrogen source", and the "anaerobic" groups where considered.
As shown in Table 1, the levels of the variables were designed and the single-factor experiments were performed as the following sequence: primary carbon source, secondary carbon source, nitrogen source, oil, and inorganic salt.
In different carbon and nitrogen sources, glucose and ammonium sulfate were found the best carbon and nitrogen source with 4 1 C: N ratio.
All algal osmolytes that R. litoralis could use as carbon and nitrogen sources in separated experiments were additionally tested combined as carbon and nitrogen source within one experiment with a final concentration of 10 mM.
For example, it was reported that P. putida can use L-alanine as a carbon- and nitrogen-source [ 68] but iJN746 cannot use this compound as a carbon or nitrogen source.
The optimal concentrations of carbon source, nitrogen source and inorganic salts were further optimized by RSM based on CCD.
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