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No exogenous carbon growth source was added.
The glyoxylate cycle is found in many β-proteobacteria, including OM5, particularly in organisms that grow well in media in which acetate and other Krebs cycle dicarboxylic acid intermediates are the sole carbon growth source.
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P. aeruginosa can utilize a wide variety of carbon sources; growth can be achieved through many different catabolic pathways in rich media despite an array of inactive genes.
Use of purified cellulose or its products like CMC as a growth carbon source is uneconomical for large-scale production of cellulases.
Bench-scale comparable results were obtained for cell growth, carbon source consumption, and antibiotic production over ∼200 h culture time, with maximum biomass reaching ∼20 g dcw/L.
To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, xylose, a hemicellulose derivate, was tested as the growth carbon source in an engineered P. putida KT2440 strain.
In this study, the possibility of using xylose as a growth carbon source and octanoic acid as mcl-PHA precusor in the controlled production of mcl-PHA by recombinant P. putida KT2440 was examined.
Carbon-containing minerals provide the carbon source, and the growth occurs over periods from 1 billion to 3.3 billion years (25% to 75% of the age of the Earth).
Initial strain characterization showed the flexibility of Synechocystis sp. PCC 6714 for utilization of different carbon sources for growth.
In contrast, wild-type Burkholderia sp. had reduced ability in utilizing 4HB-related carbon sources for growth and PHA production.
We chose P. aeruginosa (strain PAO1) as a pathogen because it can use multiple carbon sources for growth, including glucose [26].
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