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"We're looking to support the whole car cluster in Sweden.
Both car cluster and fdr gene were sandwiched between two copies of IS6100.
The putative transposase for ISSsp1 was transcribed in the same direction of the car cluster, but in the opposite direction as the interrupted IS6100.
In pseudomonads, the upper pathway genes, carAaBaBbCAcAd, are in the car cluster, which is transcribed as a single transcriptional unit [6].
In general, the genes encoding the components of the known Rieske oxygenases were found closely together in tightly regulated transcriptional units, as was observed with the car cluster in strain CA10 (Figure 5A) [26].
However, expression of genes in the car cluster was consistently decreased at both an RNA (carABCDF; fold change 0.21-0.40) and protein (carABC; fold change 0.46-0.51) level in the rsmA mutant.
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Six police cars clustered together in a lay-by off Cathcart Road in Glasgow's southside - their blue and yellow livery reflecting passing headlights almost as brightly as the high-vis jackets on police officers lining the street.
As he made his way down the front steps and over to his car, a cluster of guests watched from the porch.
The presence of IS6100 on both sides of the car gene cluster motivated further investigations using IS-based PCR. IS-based PCR
The car gene cluster (carRAaBaBbCAc) and fdr gene were accompanied on both sides by two copies of IS6100 elements, and organized as IS6100 ISSsp1-ORF1-carRAaBaBbCAc-ORF8-IS6100-fdr-IS61000.
These included a flavin containing monooxygenase, g20 from Synechococcus phage P6, gp19 a T4-like tail tube protein from the cyanophage P-SMM4, the car gene cluster from Pseudomonas resinovorans CA10, and menF from E. coli, the latter being an isochorismate synthase.
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