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After 10 days incubation, the nodule biomass of half of the plate was scraped off ensuring capturing plate diversity, suspended in 500 μl saline and mixed as described above.
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While preparing the fastener-capture plate, Massimino encountered issues with a handrail that had to be removed to accommodate the fastener-capture plate.
Once the handrail was removed, Massimino went to work attaching the capture plate, but ran into additional problems when the battery in one of his power tools failed.
As with the ACS, the STIS was not designed with the intention of servicing it while in space, and one of the major challenges was to remove a cover plate held tight with over 100 screws using a specially designed tool called a fastener-capture plate, designed to trap the screws and washers and prevent them from floating into space when removed.
The derivative samples were extracted after incubation by aqueous methanol into the capture plate.
Samples were added on filter spots placed in a 96- solvinert well plate (internal standards were placed and dried down under nitrogen before), fixed above a 96 deep well plate (capture plate).
The sample (100 μl) was applied to the capture plate for 180 minutes at room temperature (RT) (~20°C).
Blood lysates and specific probe sets were incubated in the capture plate and hybridized overnight at 55°C.
Lysates where transferred to a 96-well capture plate with target-specific probe sets, and the plate was sealed and incubated overnight at 55°C for hybridization.
Pre-amplifier and amplifier reagents were incubated in the capture plate for 60 minutes at 55°C followed by 50°C incubation of label probe with washing between each incubation.
The reaction mixture (60 μl/well) was pipetted into each assay well of the capture plate with conjugated oligonucleotides to the surface of the well.
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