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The boronic acid served both as capturing agent and the triosmium carbonyl boronic acid was employed as a secondary label (in a sandwich assay) [40].
The binder molecule for CRP was used as capturing agent in an ELISA used to detect CRP from patient sera with HRP conjugated antibodies used for detection [ 12].
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For this purpose, an aqueous alkaline solvent or various amines are used as the capturing agents.
The development of specific capturing agents for the candidate markers requires a costly production process and thorough validation.
This functionalization procedure is ideally suited to produce a stable and robust baseline signal with no loss of capturing agents.
This surface modification combined with the microfluidic system of the SPR instrument made it possible to test a panel of capturing agents in parallel with minimum sample volumes (20 μl).
Selective functionalization of the sensor gold surface with capturing agents (e.g., antibodies, ligands, and nucleic acids) enables the determination of the thermodynamics (binding constants) and kinetics (rate constants) of specific molecular interaction reactions.
However, it is technically difficult to conduct fair comparison of those devices to ours because the capture efficiency can substantially vary depending on the experimental conditions, such as flow rate, tumor cell concentrations, and types of capturing agents (antibody, peptide, or aptamer).
Through interaction on the scale of extracellular structures, increased capture agent presentations, and the ability to transition to capture agent independent methods, nanomaterials provide the advantages necessary to take these next steps in CTC research.
Her crowning achievement was to spring Cammaerts and two other captured agents from the Gestapo jail where they were awaiting execution.
Dialogical accounts potentially suffer from the objection that they are too weak to capture agents whose autonomy appears to be compromised by oppressive socialization or circumstances.
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