Our fundamental assumption is that the transcriptional signatures capture the cellular dynamics in response to the inflammatory agent.
A simple mathematical yet biological model of hippocampal CA1 pyramidal cell has been developed to capture the cellular characteristics (f-I curve, rheobase current, firing rate adaptation and rebound spiking) of those neurons in an intact preparation of hippocampus.
These time series data contain enormous amounts of information, because they are systemic as well as dynamic and capture the cellular response in a more comprehensive fashion than measurements at single time points can achieve.
As a downstream application, one can imagine capturing the cellular contents of single cells using the method developed by Lim and Abate, followed by application of the cellular material to the C3SPRs, and a final sorting component of protease activity on a microfluidics platform with high-throughput capabilities.
As shown in Figure 4A, for the SceDIP PPI network, the curve of ASModel is on top of all the other competing algorithms, which indicates that the clusters detected by joint clustering ASModel are more consistent to the curated GO terms and hence capture the cellular functionalities better.
A detailed human interactive network that captures the entire cellular network would be invaluable in interpreting cancer signatures (Calvano et al, 2005; Rhodes and Chinnaiyan, 2005).
In non-spun controls, F-actin appearing at the cortex following NE breakdown was efficiently captured at the cellular equator.
An especially exciting finding occurred when I was just starting my postdoc: it was found that the Src gene encoded by Rous sarcoma virus was actually captured from the cellular Src gene.
Hence, we designed our experiments to capture the complete cellular transcriptional response to MYC overexpression rather than try to limit our study to direct MYC target genes.
The stochastic model captures the relevant intra-cellular and inter-cellular interactions taking place in and between the eukaryotic and prokaryotic cells.
