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The basic idea is to give larger weights to the pixels in noticeable regions where the edge is strong or contrast is high, so that well captured regions are kept intact and others are changed according to the manipulated gradients.
For 192 RILs individuals, total mapped regions covered by the captured fragments were about ~7.0%% of the genome sequence and with a coverage depth of ~11.76× on average for the captured regions.
In the spring of 1993, Armenian forces captured regions outside the enclave itself, threatening the involvement of other countries in the region.
While religion no longer plays a central role as in Medieval II: Total War, it is still important in helping bring under control newly captured regions and in defining to some degree diplomatic relations between nations.
Target regions, captured regions and chromosomal positions are listed in additional file 1.
On average, the coverage was 44.5× and 95.1% of bases were called in the captured regions, yielding 51,782 substitutions and 30,290 indels per sample.
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The aspect ratio of these images were adjusted using interpolation to account for the increasing captured region which starts at 231 mm and ends at 333 mm.
Similar to our prior observations, off-target methylation accumulated along each captured region (Fig. 2b, c; Supplementary Fig. 3c), demonstrating that even multiple sgRNAs are not sufficient to prevent dCas9-cat3a from acting off-target.
In most cases, we failed to identify related Helitrons near the captured region (26 cases).
Captured product was subjected to PCR to attach molecular barcodes in a manner that allowed sequencing from either end of the captured region.
All produced aligned files were further used to calculate average read depth in 1000 bps non-overlapping windows within the captured region (Supplementary Table 2).
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