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Following automated image capture, the imaging technician quickly examined each image at low power for general image quality.
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Slides were imaged on an Olympus/3I Spinning Disk Confocal/TIRF Inverted Microscope and 6 8 random fields per coverslip were captured using the imaging software, SlideBook 5.0 (Intelligent Imaging Innovations Inc).
However, because the tails of these emission peaks (figure 2) extended within the range captured by the imaging system, they were visible to us via image capture.
Live cell images were captured using the imaging setup described above with the exception that Z-stacks (8 × 0.3 µm) were acquired every 8 min. We used exposure times of 10 ms (tdTomato) and 200 ms (GFP) for each image.
The second part of the rationale is that the largest potential error, that is differences between the anatomy captured within the imaging step and the 3D printed model, will arise at this step.
The control strip was constructed in such a way that as many color patches as possible can be completely captured by the imaging system without having to move the strip under the line scan camera.
Hb absorbs the 530 nm wavelength light, which in turn is captured via the imaging probe's light guide and a charge-coupled device camera.
A high-speed imaging camera captured the images of the BAMs as they contracted and sent the data to the computer system calculating micronewtons (μN) of force generated.
In order to optimize the imaging capture and to demonstrate the binding specificity of the galectin-3 mAb a larger in vivo experiment (repeated in triplicate) was considered.
The operator holds the manipulator with the integral probe and captures the vein in the imaging plane.
The scanner's magnet temporarily aligns these iron molecules in the hemoglobin cells, while the imaging technology captures a rapid series of "slices" — tiny cross-sections of the brain.
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CEO of Professional Science Editing for Scientists @ prosciediting.com