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We targeted 594 Kb of DNA sequence from the coding regions of genes that were also sequenced in the 1000 Genomes exon pilot project[12] by solution based hybridization capture (Table S2).
Genetic analyses confirmed that the majority of juvenile sockeye on the north coast were from the Skeena, Nass, and adjacent watersheds (98.3% combined), and thus they were unlikely to have been influenced by salmon farms further south before capture (Table 1; Figure 1).
It is recommended that the guidelines for genetic capture (Table 1) are followed for this translocation.
Overall prevalence for the two herds was estimated at 89.5% (77/86) at capture (Table 1).
The statistics for each library indicate efficient exome capture (Table 2).
Six of eight samples underwent successful enrichment using both RainDance emulsion PCR and SureSelect hybridization capture (Table 2).
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A total of 221 sharks from 7 species were captured (Table 1).
Note that swarming ants (winged males and queens) and termites were also frequently captured (Table 1).
Galapagos (36.2%), gray reef (Carcharhinus amblyrhynchos, 25.8%) and tiger (Galeocerdo cuvier, 20.4%) sharks were the numerically dominant species accounting for 82% of all sharks captured (Table 1).
In all, 120 rodents representing nine species and seven opossums (two species) were captured (Table 5).
In several trap locations more than one individual of the same species was captured (Table 1).
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