Exact(9)
Reads obtained from sequence capture were mapped to their respective reference targets (consensus sequences of contigs from the combined assembly) resulting and 403 SNPs in 134 contigs had capture reads overlapping their respective positions.
Using 0.95 sequence similarity, the capture reads were mapped to the reference contigs in order to place individual reads at previously called SNPs and SSRs using Genomics Workbench 4 (CLCBio, Aarhus, Denmark).
In order to capture reads containing partial intron sequences, a comprehensive panel of references was designed by appending 50 bp of intron sequences extracted from the IMGT/HLA database to sequences from both ends of a reference exon.
For each contig that was mapped to the reference genome, we extend it by the length of a read (e.g., 100 bp) on either end, so that we can capture reads that align only partially to the contig.
From the contigs assembled, two largest contigs with similar GC percentage were used as baits to once again capture reads belonging to the chloroplast sequence from the full error corrected PacBio reads using the GMAP aligners, set at default.
Of these 17 SSRs, five SSRs were polymorphic in repeat number, four SSRs contained SNP polymorphisms in one or more repeats, and five SSRs did not have any polymorphisms detected in the sequence capture reads.
Similar(51)
With a few simple hypotheses, SHARE is able to capture reading eye-movement patterns of beginning readers and proficient adults, and to reproduce well-known psycholinguistic effects.
We also generated a reference from MBD2-seqs wincorporatingorating MP and MeDIP-capture reads.
Compared with the low coverage of MP reads (4×) and the even lower coverage of MeDIP-capture reads (0.84×), which only led to a low increase in mapping efficiency, the best results were obtained using MBD2-captured reads (coverage: 7.3×) and MEBS-PE reads of the same sample material.
These flanking and intronic sequences facilitated reconstruction of genomic sequence from the de novo assembled captured reads.
Captured reads were obtained by using the Roche 454 GS FLX sequencer and assembled by using the Newbler 2.7 software in the genomic mode (Roche Diagnostics).
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