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Under inducing conditions (LB broth with 0.8% bicarbonate in a 5% CO2 atmosphere), protective antigen (PA), lethal factor (LF) and edema factor (EF) were synthesized [21] and immunostaining of bacteria with the monoclonal antibody F26G3 [50] confirmed the production of an anthrax-like capsule (data not shown).
The capsule data were transmitted to a recorder attached to the abdomen.
The individual capsule data revealed the within and between batch consistency and statistical distribution across the specification range.
The system includes a wireless motility capsule, data receiver, and data analysis software GIMS Data Viewer v.1.4 and SmartPill MotiliGI, which are provided by the manufacturer.
Because TCAs turn into ventral telencephalon and extend dorsolaterally through it to neocortex, we examined at E13, E15, and E17 Slit expression within ventral telencephalon relative to the TCA pathway, the internal capsule (data not shown).
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Similarly to AGFP homozygous larvae, both hypomorphic (cactus[E8]/cactus[IIIG]) and null (cactus[E8]/cactus[D13]) mutant combinations [20] together with AGFP[23]/+ showed GFP fluorescence in aggregating hemocytes and especially in the outer border of melanotic capsules (data not shown and Figure 5G).
Fourier transform infrared spectra were collected on a Varian 1000 spectrophotometer and used to check the composition of the capsules (data not shown).
This was particularly helpful for analysis of the capsule-filling data, since capsule weight variability displayed a threshold behavior as a function of the mixture fraction.
This system comprises an implantable capsule, a data logger and wireless energy transmission system.
Habitat and landscape scale fragmentation predictor variables were used to build parsimonious models that explained capsule set data collected over 2 years.
However, a mutation in wbtK resulted in loss of high mass O-antigen expression but did not affect capsule expression (data not shown).
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