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We laser captured these areas by separate under direct microscopic visualization by melting of the selected regions onto a thermoplastic film mounted on optically transparent LCM caps using the Arcturus XP equipment (Arcturus Engineering, Mountain View, CA, USA).
After drying of slides, epithelial and stromal cells were captured on individual caps using the Veritas 704 apparatus (Arcturus).
The reactions were set up on ice in 0.2 ml optical tubes with caps, using the Quantitect SYBR Green PCR Kit (Qiagen, Hilden, Germany).
Pulmonary inflammatory and hematologic responses of canines were studied after exposure to concentrated ambient particles (CAPs) using the Harvard ambient particle concentrator (HAPC).
OM and LSC motor neurons, identified by staining, anatomical location, size and morphology, were isolated on Capsure Macro LCM caps using the Arcturus PixCell II laser capture microdissection system (Arcturus Bioscience).
Spinal motor neurones, identified by staining, anatomical location, size and morphology, were isolated on Capsure Macro LCM caps using the Arcturus PixCell II laser capture microdissection system (Arcturus Bioscience, Mountain View, CA).
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MLS salaries are limited by a salary cap, but each team is allowed to go over the cap using the "designated player" rule, which allows them to bring in big names.
The N-terminals of the residues were capped with acetyl groups and the C-terminal ends were N-methyl capped using the geometry of the cleaved neighbouring residue as a vector to place the capping group.
The additional TMS condition, EEG location POz could be marked on an EEG cap using the international 10 20 system, therefore no anatomical determination was needed.
EEG data were collected from a whole-head forty electrodes embedded in a cap using the international 10 20 method of electrode placement.
The electroencephalogram (EEG) was obtained from 64 active Ag/AgCl electrodes (Biosemi ActiveTwo system), digitally recorded at 512 Hz, mounted on an elastic cap using the International 10/20 system.
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