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Persoff et al. (1991) studied two-phase flow for gas and liquid in a rough-walled fracture with controlled injection rates and pressures using Hassler and Brunner (1945) method and porous end caps to avoid capillary end effects and to maintain uniform capillary pressure across the entire fracture plane.
Induction experiments with 20 µM toluene were carried out in 2 ml glass vials closed by Teflon-lined caps to avoid evaporation.
The material was immediately transferred into sterile 1 l bottles, which were subsequently filled with anoxic sulfidic groundwater derived from the column inflow, and closed with air-tight screw caps, to avoid oxidation processes caused by oxygen.
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Discs were then placed in NMR tubes which were closed with a 2-cm long Teflon cap to avoid water loss during measurements.
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