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Strobilurin resistance in M. nivale/majus was analysed in selected wheat samples from 2003 to 2007, selected barley samples from 2007 as well as in historical samples from 1957 to 2000 using CAPS analysis to detect the G143A substitution.
My focus everywhere and always is on a technique I developed after teaching at the University of Virginia and James Madison University for many years and then working with Heartlands Bill Nasgovitz on micro caps analysis.
cDNA CAPS analysis was performed for 15 of 30 genes.
Loci with the prominent variant that contained the six base restriction site were chosen for the CAPS analysis.
Four sites were identified, and primers were designed to amplify these regions for CAPS analysis (Supplementary Table S4).
In cases where one allele of a homoeolog was lost, CAPS analysis might not detect these losses.
Similar(31)
At present, there is an immense experimental effort for promoter identification and characterisation using techniques such as gene reporter assays, chromatin immunoprecipitation, oligo-capping, cap analysis of gene expression (CAGE) and 5′ end serial analysis of gene expression (SAGE) (Suzuki et al., 2001; Shiraki et al., 2003; Hashimoto et al., 2004; Kim et al., 2005; Cooper et al., 2006).
The software takes into account known transcripts as well as recorded CAGE (cap analysis of gene expression) tags that correspond to the 5'-end of capped transcripts and thus provide a picture of TSS.
Fig. 9 Protein cap analysis.
Macrostructural sleep and CAP analysis were also performed.
The immunomagnetic assay showed promising potential in rapid screening field for CAP analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com