The latter is electronically conducting thus being employed as electrode material and contact material in super caps [1].
To investigate the intracellular dynamics of CAPS-1, we introduced CAPS-1 fused to enhanced yellow fluorescent protein (EYFP) (CAPS-1-EYFP) in CAPS DKO neurons.
Furthermore, in CAPS DKO neurons rescued with CAPS-1-EYFP, DCVs and CAPS-1-EYFP only rarely traveled together.
CAPS-1 (KIAA1121-Kazusa DNA) was sequence verified and cloned as CAPS-1-ires-EGFP and EYFP-CAPS-1.
CAPS-1-EYFP fusion protein replaces CAPS-1 in DCV secretion and synaptic transmission.
Like endogenous CAPS-1, CAPS-1-EYFP was present in puncta.
In addition, we observed, again similar to endogenous CAPS-1, synapses without detectable CAPS-1-EYFP and CAPS-1 rich domains that did not co-localize with synapses.
CAPS-1 displayed a different expression pattern than Munc13-1.
CAPS-1 domains were also found at extra-synaptic sites.
As for endogenous CAPS-1, the majority of CAPS-1-EYFP co-localized with the live-synaptic marker synapsin-ECFP.
CAPS-1-EYFP expression in DKO neurons restored DCV secretion, but CAPS-1-EYFP and DCVs rarely traveled together.
