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A comparison of the cost and high-throughput capacity of sequencing technologies indicated that 454-Roche pyrosequencingenerateded far more sequence data per run at a much lower cost (30 times) than conventional dye-terminator sequencing [ 10].
With the increasing capacity of sequencing technologies, the lag between sampling and sequencing viral genomes is expected to decrease, making earlier parameter estimation feasible in future epidemics and before alternative types of data become available.
On the other hand, NGS technologies have more throughput because they have 100 times more capacity of sequencing than Sanger method.
However, that started to change towards the end of the HGP, when the capacity of sequencing instruments began to exceed the rate at which new samples could be prepared for sequencing.
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To minimize the cost of targeted assays, we utilize a small percent of sequencing capacity available in runs of GBS libraries to "spike" amplified targets of a priori alleles tagged with a different set of unique barcodes.
Increasing capacities of sequencing technologies combined with the complexity reduction allow for multiplexing of 48 to 96 samples.
The advent of NGS technology has increased sequencing capacity and lowered the cost of sequencing [ 21], making it an appealing alternative to Sanger sequencing for genetic testing.
To make the most of the capacity of the sequencing plate, this library was mixed with another of BRCA amplicons to be sequenced in a single run.
Moreover, NGS is suitable for transcript profiling, combining the high throughput of serial analysis of gene expression (SAGE) with the functional annotation capacity of EST sequencing [ 30].
With the super high capacity of shotgun sequencing facilities it might already now be possible to obtain WGS data for all teleost fish species.
The increasing capacity of DNA sequencing technologies has permitted substantial increases in genomic resource availability for several legume crops that had been previously underdeveloped.
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