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For passaging, 1 × 10 cells were replated in 25 cm flasks in the same condition as in the culture, and the cells were analyzed for their capacity for viability, differentiation, phenotype, and karyotype as well as for culture behavior.
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While no introduced population has demonstrated a capacity for long-term viability, one augmented population has flowered and produced viable fruits.
The most important factors for developing microbial starters include maintenance of cell viability, capacity for long-term storage and the drying method used.
Tu et al. (32) reported that the C-terminal region of HBx is crucial to its transcriptional function and that, in tumor tissues, most HBx natural mutants have lost their capacity for controlling cell proliferation, viability and transformation.
Importantly, aggressive breast cancer cells of luminal B, HER2-overexpressing and triple negative subtypes show dependency on CPSF6 for viability and tumorigenic capacity.
This is likely a result of the inbreeding strategy, which inadvertently selects for sporulation capacity, spore viability, and mating ability.
After two additional washes in PBS, cells were assessed for viability, proliferation, or migratory capacity.
Radiation cell kill means loss of capacity for sustained proliferation (loss of cell viability) as a consequence of radiation-induced injury.
Isolates that were used for a next round of shuffling were always tested for sporulation capacity and spore viability.
We also monitored the biodegradation of silica hydrogel shell over time, and evaluated the bioactivity of the CSP cells underneath the silica hydrogel by measuring their viability and evaluating their capacity for migration and proliferation.
In order to determine whether the glycyl-glycyl endopeptidase enzymatic activity of LytM was specifically required for its capacity to restore viability to WalKR-depleted cells, we used site-directed mutagenesis to inactivate its catalytic site.
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