For example, T7 and E. coli DNAPs are capable of strand displacement synthesis in the presence of SSB with rates comparable to their replication rates (Yuan and McHenry, 2009; Pandey and Patel, 2014).
While both the WT (dimeric) and W187A (monomeric) PR8 NS1 EDs are capable of forming strand-strand dimers within the confines of a crystalline array, such dimers do not appear to exist in solution.
Only when BtsIA is combined with BtsIB do they form a restriction endonuclease capable of double-strand cleavage (GCAGTG(2/0))[4].
Therefore, we modeled a typical cDNA microarray tumor-classification analysis and compared the results from conventional double-stranded cDNA capture probes with single stranded cDNA capture probes capable of monitoring opposite strands of each cDNA independently.
To reduce potential off-target mutagenesis by wild-type Cas9, homology- and structure-guided mutagenesis of Streptococcus pyogenes Cas9 catalytic domains has produced "nicking" enzymes (Cas9n) capable of inducing single-strand nicks rather than double-strand breaks.
Topoisomerase I is capable of introducing single strand breaks in DNA, while topoisomerase II can break both strands.
These biosensors are, in fact, obtained by immobilizing well-defined sequences of single strands DNA on an electrode surface that in specific conditions are capable of binding DNA strands of pathogenic microorganisms.
Exposure of mammalian cells to chemicals was performed for 60 min on ice thus allowing identification of direct acting substances capable of inducing DNA-strand breaks.
It is concluded that silica causes bigger effect size and is more capable of breaking DNA strands.
Online this week in Nature, they show that by using a technique called test-tube evolution they were able to generate ribozymes capable of assembling RNA strands of the opposite handedness in the presence of a mixture of D- and L-RNA nucleotides.
