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With current sequencing technologies capable of generating read lengths up to 2 × 300 bp (Illumina MiSeq; see http://www.illumina.com/systems/sequencing.html), sequencing of flanking intronic regions captured or amplified by exonic probes or primers is becoming straightforward.
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The Pacific Biosciences RSII system is capable of generating large reads, and with sufficient coverage, can generate high quality single contigs for bacterial genome sequences.
We used single-molecule real-time sequencing (SMRT) implemented in the PacBio RS platform (Pacific Biosciences, Menlo Park, California, USA) because it is capable of generating long reads (Wei et al., 2014).
The first 454 Genome Sequencer was capable of generating approximately 200,000 reads of 110 base pairs (bp) in length (the current maximum read length is 1000 bp).
All simulation runs indicated that MEGAN generally is capable of binning the majority of all generated reads correctly.
The new 454 GS FLX Titanium is capable of generating over a million reads of 400 bases with reduced error [ 4].
GemSIM, or General Error-Model based SIMulator, is a next-generation sequencing simulator capable of generating single or paired-end reads for any sequencing technology compatible with the generic formats SAM and FASTQ (including Illumina and Roche/454).
Current technologies (Ronaghi et al., 1998), e.g. the Illumina and SOLiD platforms, are capable of generating hundreds of millions of short reads in a single run (Applied Biosystems, 2008a; Bennett, 2004).
Next generation short-read sequencers are now capable of generating hundreds of gigabases of sequence data per run.
Overall, we believe the analysis benefited considerably from the use of the much longer reads that 454 sequencing is capable of generating.
We have developed a DVD-based read-out system capable of generating large sets of cantilever data for vapor and liquid phase detection of 2,4-dinitrotoluene (DNT).
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CEO of Professional Science Editing for Scientists @ prosciediting.com