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Moreover, they had not yet developed Na+ currents capable of generating action potentials.
At all ages studied, however, motoneurons are still capable of generating action potentials.
Electrophysiological recordings showed that treatment of hESC with SPIE induced differentiation of neurons that were capable of generating action potentials and forming functional synaptic connections.
It is interesting to note that although riluzole largely abolished the evoked SMPOs and the repetitive firing, these DRG neurons still responded to the same depolarizing current pulses with a single action potential at the initial phase of the current pulse, indicating that these cells are still capable of generating action potentials in the presence of riluzole (Fig. 3C).
All cESC-derived and primary neurons displaying synaptic activity exhibited characteristic Na+ and K+ mediated inward and outward currents when stepped from −100 to 0 mV in voltage-clamp mode (ie. were capable of generating action potentials), while none of the non-active cells displayed these characteristic currents.
These data indicate that human ESCs can be differentiated into granule neurons capable of generating action potentials in vitro.
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The unregulated flow of ions alters the membrane potential such that it becomes capable of generating a multitude of action potentials at the site of injury.
First, CaV1.3 channels activate at relatively hyperpolarized membrane potential, as negative as -70 mV in immature IHCs [ 100, 108], indicating that they would be capable of generating both the spontaneous action potentials in immature IHCs and the fast synaptic response of mature hair cells [ 84].
They typically agree that practical reasoning is capable of generating new motivations and actions.
MSC is capable of generating methylselenol endogenously through the action of β-lyase or related lyases [ 38].
MSC is a stable selenoamino acid that is capable of generating methylselenol endogenously through the action of β-lyase or related lyases [ 31].
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