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Labelled oligonucleotides were incubated with nuclear extracts from COS-1 cells transfected with pSG5-AR and used to determine their capabilities to bind to nuclear proteins.
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Given their small size, high physicochemical stability, facile genetic manipulation, and capability to bind epitopes inaccessible to conventional antibodies, sdAbs are ideal antigen-binding elements [ 7].
Interestingly, while ΔUBA had wild-type capability to bind CUL2, the ΔUIM truncation caused a pronounced reduction in CUL2-binding.
MIBE displaced the radiolabeled E2 in a dose-dependent manner indubitably demonstrating its capability to bind to ERα in a direct fashion, although with a lower binding affinity in respect to E2 and OHT as 10 μM MIBE induced approximately 40% displacement of [3H]E2.
Furthermore, we analyzed different yeast strains for their capability to bind E. coli F4 and Salmonella Typhimurium.
Aliquots of the different time-points samples were tested for their capability to bind LM-111 by ELISA.
In contrast the NKX2.1 mutation did not show any transactivation ability due to the loss of the capability to bind to DNA.
These results suggest that the cell wall of ral1 mutant root tips had a reduced capability to bind Al, resulting in increased resistance to Al.
Anoxia accelerates eutrophication, because in low oxygen conditions, iron (Fe) is reduced and loses its capability to bind phosphorus (P) (e.g. Boström et al. 1982).
Summarizing, the present study demonstrates a variation among different yeast products concerning their capability to bind E. coli F4 and Salmonella Typhimurium.
Thereby the resulting R- and S- sensors showed a very high capability to bind selectively the target enantiomer from a racemic mixture.
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