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Then, the transwell assays were performed to assess the effects of Capn4 knockdown on capabilities of invasion and migration in NPC cells.
However, we noted that even after knock-down of HPA, the gastric cancer cells still possessed the capabilities of invasion, metastasis, and angiogenesis.
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Although early diagnosed melanoma is curable with surgical excision, up to 20% of patients will develop metastatic tumors due to its high capability of invasion and rapid metastasis to other organs [3], [4].
Indeed, melanoma has a high capability of invasion and rapid metastasis to other organs.
Similar to VM, EMT is another phenomenon of acquiring the capability of invasion and metastasis by changing into an embryonic genotype.
On the other hand, the HPV-negative tumours develop EMT-like and, therefore increased capability of invasion and progression, leading to worse prognosis and poorer outcome.
Although early melanomas are curable with surgical excision (Balch et al, 2001), up to 20% of patients will develop metastatic tumours due to its high capability of invasion and rapid metastasis to other organs (Houghton and Polsky, 2002).
Since our previous data indicated that cell proliferation could be inhibited via targeting PLK1 by mir3686, we want to know if PLK1 inhibited by mir3686 could reduce the capability of invasion for PANC1 cell.
To further evaluate the capabilities of tumor invasion and metastasis of LC-CD133+ treated by different regimens, we injected 104 LC-CD133+-GFP cells from each treatment groups into the tail vein of SCID mice.
In addition, LIS1 silencing reduced capabilities of cell invasion both in HCCC-9810 and CCLP1 cells compared with control cells (P <0.05).
To evaluate the capability of cell invasion, the Transwell cell invasion assay had been conducted as previously described with modifications [ 20].
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