The resulting fragments were end repaired using the End-It™ (Epicentre) kit, methylated to protect EcoP151 sites, and ligated to CAP adapters.
CAP adapters were ligated in 500-μL reaction volumes of New England Biolabs Quick Ligase reaction mix.
Purified restriction fragments were attached with sticky CAP adapters that were modified from standard SOLiD CAP adapters [5′- CGC TGC TGT AC -3′ (positive strand); 5′- ACA GCA G -3′ (negative strand); 100 μM].
The fragmented DNA was end repaired using the End-It™ kit (Epicentre Biotechnologies, MA, USA), and ligated to LMP CAP adapters for 2 × 50 bp libraries and EcoP151 for 2 × 25 bp libraries.
Following CAP-adapter ligation, DNA fragments were purified with phenol chloroform:isoamylalcohol (PCI, pH7.9) by gentle mixing and centrifugation in MaXtract high-density tubes (QIAgen, 1.5mL, #129046).
That's as much plot as you'll get here, but a tip of the cloth cap to the adapter, Arthur Perlman, and to the producers, David Richenthal and Jack Viertel, for bringing some clarity and crispness to the vexed book, by Fred Saidy and Harburg.
That's as much plot as you'll get from me, but a tip of the cloth cap to the adapter, Arthur Perlman, and to the producers, David Richenthal and Jack Viertel, for bringing some clarity and crispness to the vexed book, by Fred Saidy and Harburg.
On the other hand, a 50 mm f/1.2 Nikon objective that provides a field of view of 15° is also attached to the Atik 11000 CCD via an adapter cap.
Pressure regulated nitrogen gas was connected to a syringe reservoir through an adapter cap (KDS503S6, Techni-Tool Inc ., and the syringe was connected to the sub nanoliter dispensing valve by tubing (Tygon® tube, Fisher scientific Inc).
Unscrew the adapter cap (Red) by turning counter-clockwise.
Remove the hoses and adapter cap to the brake bleeder pump and set aside.
