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Although we cannot exclude with certainty the role of t-Ag and other T'- proteins, based upon our results it is reasonable to speculate that T-Ag may be the primary mediator of a metastatic phenotype in colon cancer cells, and some of these effects may directly or indirectly be influenced by the activation of AKT/MAPK pathways [44], [45].
However, we cannot exclude with certainty that some combinations might lead to a different result.
Therefore, we cannot exclude with certainty, that some participants had early cartilage or ligamentous changes or deviations from neutral alignment.
A limitation of this study is that we cannot exclude with certainty that the spouses of probands, their siblings and offspring in the LLFS have familial longevity.
Although these samples originate from a non-breeding facility, we cannot exclude with certainty that these hybrids have been produced in captivity [ 25], such that our inference about naturally occurring gene flow and hybridization should be considered preliminary.
Second, we did not record counsellor counselee phone or mail contacts, so we cannot exclude with certainty that topics that were not discussed during initial visits at the clinic were actually not discussed with counselees who were seen once only.
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We cannot, therefore, exclude with certainty that reverse causality played a role.
Contamination with maternal blood cannot be excluded with certainty based on the analysis of IgA in cord blood.
In the remaining 12 patients the possibility of concomitant TB infection cannot be excluded with certainty and thus misclassification bias may explain the detection of LAM antigen, at least, in some of these patients.
This means that an adenocarcinoma cannot be excluded with certainty.
Nevertheless, this mutation cannot be excluded with certainty as a cause of PD when in combination with α-synuclein.
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