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Natural francium cannot be isolated in visible, weighable amounts, for only 24.5 grams (0.86 ounce) occur at any time in the entire crust of Earth.
Our 12-bed ICU is made of three units of 2 twin bedrooms each so that recommendations on contact precautions cannot be thoroughly followed, since patients cannot be isolated in a single room.
If an organism cannot be isolated in the laboratory, however, it cannot be observed morphologically or characterized biochemically.
As another example, dominant mutations that are rescued by a wild-type maternal genotype cannot be isolated in the F1 generation, but can be sought among F2 animals.
Therefore, they cannot be isolated in sufficient numbers from a small volume of blood from most patients.
For example, many patients recover because of complex, synergistic or idiosyncratic reasons that cannot be isolated in controlled environments.
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Such studies may be justified when (1) safety cannot reasonably be predicted from other evidence, (2) reasonable hypothesis for adverse effects are postulated, (3) the compositional component in question cannot be isolated or enriched in an active form for inclusion in animal feeding studies, and (4) reasonable multiples of exposure can be accomplished relative to human diets.
Because specific environmental factors cannot be isolated and controlled in the field, laboratory studies will be the best way to test the effects of specific changes in temperature, humidity, or physiological stress on host, vector, or pathogen populations.
Also, the expression of any genetic effects cannot be isolated from the environment in which organisms are reared [47], [48].
Given these complications of size, the post-infection parasite cannot be isolated from host cells in sufficient numbers, nor can sufficient post-infection parasite protein or RNA be obtained for most downstream molecular applications.
In addition, missense alleles cannot be isolated and large deletion events may result in the loss of function from more than one locus simultaneously.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com