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We recently found that challenging HCECs with either purified flagellin or invasive strains of P. aeruginosa resulted in an up-regulation IL-1β at both mRNA and protein levels but not in the secretion of IL-1β (Yu et al unpublished results), suggesting that either flagellin cannot be internalized or Ipaf-caspase axis is not functional in HCECs.
We demonstrated that large, but aggregated or amyloid fibrils of hPrP90-231 cannot be internalized and cause cell death.
In this regard, the previously noted potency of curdlan as an innate stimulus appears to relate to its ability to form large particles that cannot be internalized.
Importantly, the Lyp1-K10XR proteis is efficiently delivered to the PM but cannot be internalized or degraded by the heat-induced endocytic response.
It is important to note that the Lys24AlaMet27Ala, non-CALM binding mutant of VAMP8 cannot be internalized but, as we show in this work, is able to form SNARE complexes with its cognate plasma membrane SNARE partners.
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The problem cannot be solved by consultants alone but must be internalized by the enterprise users in order to competently establish and maintain process automation.
Clare's still internalizing whatever is to be internalized.
While MIF cannot be taken up by GFP transfected cells (data not shown), it can be internalized by GFP-CD74 transfected cells.
The result indicates that although cell surface-bound Notch can be internalized in awd mutant cells in the presence of Rab5CA, it is not processed and cannot enter late endosomes.
It needs to be internalized companywide.
Thus, the externality of trade can be internalized.
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