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The 15 downregulated putative sHSP belong to class I, class II, class III, chloroplastic endoplasmic reticulum, or cannot be assigned with confidence to a known class.
Although the identity of every WGS contig cannot be assigned with confidence, evidence suggests that the little skate has both a POU2 and a POU5F1 ortholog, while the elephantfish has only a POU2 ortholog.
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Because of their short sequences, ELMs cannot be assigned with high confidence.
Because WS0064_K01 and WS0061_H08 are branched between mitochondrial group I and chloroplastic sHSP within the large clade consisting of both mitochondrial and chloroplastic sHSP, they cannot be assigned with high confidence to either class.
This enabled the rules to be assigned with confidence.
Finally, markers that failed to be assigned with confidence to only one LG were not considered in further analysis.
Unlike glycosidic linkages and NIR spectra, epitopes can almost always be assigned with confidence to particular polysaccharide structures.
The plastid localization of the At5g39790 homologues, which currently cannot be assigned chloroplast transit peptides with confidence, will require experimental investigation.
Indeed, some of the sequences cannot be assigned to a definite group with high enough confidence.
Yiduxylon cannot be assigned to the Buteoxylonales with their undivided leaf trace and T-shaped petiolar vascular supply.
Without reference to the known dideoxy sequences, as would be the case for de novo microarray sequencing of an unknown genome, low-confidence calls cannot be assigned a priori as either correct or incorrect.
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