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Since very low-expressed transcripts cannot be assembled by any assemblers, the total number of transcripts (18 180) in all components was less than the total number of expressed transcripts (22 4022.
If it cannot be assembled as a sub-assembly, it will be divided into several sub-assembles or parts to adjust the product hierarchical graph.
However, overlapping transcript alignments that have different introns in their region of overlap, due to a splicing variation, are found incompatible and cannot be assembled together, and hence occupy distinct maximal alignment assemblies.
However, it is currently unclear whether this reflects true biological trends or whether it is an artifact resulting from the assembly or binning process, in which highly heterogeneous sequences cannot be assembled or clustered into MAGs.
Other de Bruijn graph assemblers such as Oases [ 46] and ABySS [ 55] are promising but are currently not suitable for most non-normalised 454 pyrosequencing de novo transcriptome assembly projects because many transcripts have very low coverage that cannot be assembled reliably.
Although current sequence assemblers successfully merge the overlapping reads, often several contigs remain which cannot be assembled any further.
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Tempting benefits packages cannot easily be assembled to keep the restless in place, for shareholders remain beady-eyed about labor costs.
Short reads of alpha-gliadins cannot readily be assembled as the members of the gene family are very similar.
However, gaps can occur if reads that would typically be assembled into one contig cannot span a large repeat area.
While one cannot drink beer from the keg while the stool is attached, the kit can be assembled and disassembled without harming the keg shell.
With unpaired sequencing, repetitive sequences that are longer than the average read length (e.g. prophage, rRNA gene cassettes, transposons) cannot be unambiguously assembled and force de novo assembly algorithms to terminate regardless of the fold sequence coverage.
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