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The screening of potential aptamers and the binding affinity of the successful aptamer candidates were done by flow cytometry using biotin-labeled aptamer, and the signal was detected with streptavidin-R-PE conjugate (0.5 mg R-PE at 0.25 mg/mL SAV, Invitrogen) or streptavidin-PE-Cy5.5 (.2 mg/mL).
Scoring and ranking of loop candidates were done using the FoldX stability command that estimates the free energy of folding (ΔΔG).
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Some of the explanation also lies with which candidates were doing the advertising.
The same night the two candidates were doing stand-up comedy at the Waldorf Astoria, super-surrogate Clinton was sharing a stage at Cuyahoga Community College in Parma, Ohio, with Bruce Springsteen – and that had to have been fun.
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"I... would like to clarify that all political activity undertaken by Barclays USS employees, including personal fundraising for specific candidates, is done so in a personal capacity, and not on behalf of Barclays," he wrote.
The selection of candidates was done through six rounds of examination (which took 3 months) and managed by two different organisations (in the Hungarian system, these positions are supervised both by the Ministry for Foreign Affairs and the National Office for Research and Technology).
The computational prediction for U1-snRNA candidates was done using RNAbob (Materials and Methods).
The initial assessment of potential aptamer candidates was done with RCA reaction products obtained from sequencing in different 96-well plates.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com