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Furthermore, the analysis revealed a kinase-kinase subnetwork and candidate substrates for CMGC kinases.
Each of these methods has identified interesting candidate substrates, some of which have been validated using various biochemical tests.
By using two independent detection approaches, we identified novel candidate substrates for SETD6, and verified that all targets tested in vitro and in cells were genuine substrates.We describe a novel proteome-wide methodology for the identification of new PKMT substrates.
The quantitative rate of hydrogen oxidation on the candidate substrates was determined for different substrate potentials from SECM approach curves by fitting to a theoretical model.
Bioinformatic analysis of the consensus phosphorylation motif identified several novel candidate substrates that potentially function in neuronal pathophysiology.
Furthermore, the interacting partners we have identified are candidate substrates for the kinase activity of Rio2.
To confirm that the restricted motif yields biologically relevant candidate substrates we performed multiple biosemantic analyses.
A few candidate substrates were identified as being responsible for Cdk2 function in centrosome duplication.
Of these, 35 are contained in our set of 91 candidate substrates (38%).
Therefore, we believe that proteins containing sequence matches within the disordered regions are very likely candidate substrates.
Application of these criteria to the compounds in the BiSSCat database showed that 1,912 known substrates have more than 10 related structures that were, therefore, candidate substrates, 1,166 known substrates had between 1 and 10 other candidate substrates, and 934 had no alternative candidate substrates.
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