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These analyses helped identify not only better candidate pathways, but also superior chassis organisms that can be used for metabolic engineering of the candidate pathways.
GenGen got 133 candidate pathways from Group B, but none from Group A. Furthermore, SRT provided 10 candidate pathways from Group A and 5 candidate pathways from Group B, and there was no overlapped pathway between Group A and Group B. Comparison among different PBA tools for the same GWAS dataset was conducted.
By inputting SNP P-value list, i-GSEA4GWAS v2 generated 31 candidate pathways for Group A and 31 candidate pathways for Group B, which shared 12 pathways between Group A and Group B. The calculation of gene-set enrichment score was similar between i-GSEA4GWAS v2 and GenGen, but GenGen used genotype data as input and permutation was conducted at phenotype level.
These studies highlight the interplay between host and parasite lipid metabolism and provide a comprehensive analysis of P. falciparum lipids with candidate pathways for drug discovery efforts.
The best candidate pathways are then engineered within a metabolic network of microorganisms that serve as synthetic platforms for synthetic biology.
One of the candidate pathways is through NALP1.
Until very recently, candidate gene association studies have focused on genes in a few candidate pathways.
Future epigenetic studies should be conducted within the candidate pathways and processes described.
The MAPK pathway was one of the top candidate pathways to be affected by Aβ-mediated down-regulation of miRNAs.
Indeed, a large-scale pathways-based association study in ALS recently failed to exhibit significant findings for other candidate pathways [60].
Alternative estrogen-dependent pathways mediated by ERβ or GPR30 are potential candidate pathways since estrogen is known to interact with these receptors and are currently under investigation.
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