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These genes were used to normalize expression of the candidate maternal effect target genes using the standard curve method.
When restricting the data set to BMP components or transcription factors, we readily identified additional candidate maternal expression differences in both gene groups.
To exclude batch effects as a potential cause of maternal effects, we used qPCR to validate candidate maternal effect target genes on an orthogonal platform.
Chloroplast DNA haplotypes of several hybridogenous accessions (H. plumulosum, H. olympicum/H. gymnocephalum, H. mixtum) were unique and fairly divergent from others given the low overall variation of this marker, i.e., a candidate maternal parent is missing.
Based on a review of records from the Research Registry for Neonatal Lupus [ 6], however, we have recently learned that incomplete AV block can progress postnatally despite the clearance of the candidate maternal antibodies from the neonatal circulation [ 7].
Hence the Hydra Myc2 protein could represent a candidate maternal factor in the oocyte like in Xenopus, where myc has been detected as stable maternal mRNA (Godeau et al., 1986; Taylor et al., 1986), and the accumulation of myc2 mRNA may support cell division in the early Hydra embryo.
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Fau modifies apoptosis-controller Bcl-G, which is also a key target for candidate oncoprotein maternal embryonic leucine zipper kinase (MELK).
We suggest that these proteins are excellent candidates as maternal effect proteins.
Moreover, detailed study of the expression of some of these genes revealed promising candidates for maternal effect genes in the chicken.
For all of these hemizygous baz embryos, maternal candidate interacting gene dosage is reduced by half, and for half of them, the zygotic candidate interacting gene dosage is also reduced by half (Fig. 1A).
Our attempts to amplify the predicted novel candidate gene from maternal and post-somitogenesis zebrafish cDNA libraries using 2 different primer pairs failed, while mkp3 cDNA was readily amplified in parallel PCR reactions (data not shown).
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