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Figure 1 shows a candidate differential exon skipping event identified by MADS+.
Candidate differential gene expression was developed by querying the database for read counts which were different in bi-directional comparisons between each of the tissue pairs.
The transcriptional composition differences in the apical and basal cells and possible function of some candidate differential transcripts are further discussed.
Therefore, this minor limitation mainly hampers abundance comparisons across different proteins, and estimation of absolute protein amount, which were not necessary for our determination of candidate differential biomarker status.
Finally, for candidate differential splicing events, MADS+ plots the background-corrected intensities of individual probes as well as the estimated gene expression indexes, allowing users to directly inspect the evidence for splicing differences between sample groups (see Fig. 1C for an example).
Three separate sets of patient samples were obtained to establish the genome-wide methylation profiles of the plasma cfDNA by MethylCap-seq, screen and identify candidate differential methylation regions (DMRs) in tissue DNA by MSP/qMSP and validate candidate DMRs in plasma cfDNA by Multiplex-BSP-seq.
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Recruitment, therefore, was a candidate for differential roles of Adr1 and Cat8.
We revealed IL-8 as a new candidate for differential regulation by HIF-1α and HIF-2α.
For this reason, we required mapped genes to have an RPKM ≥ 10 in order to be considered as a candidate for differential expression.
For a gene to be selected as a candidate for differential splicing, at least one probe has to fall outside of the limits of the marked threshold.
To identify TB-specific candidate miRNAs, differential expression of miRNAs between patients and the healthy controls were required to meet two criteria: (1) CT values <35 to enable reliable detection, and (2) miRNA levels exhibiting ≥2-fold difference between the patient and control groups [ 25].
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CEO of Professional Science Editing for Scientists @ prosciediting.com