The questionnaire for the district cervical cancer coordinators included questions on how services are delivered, who provide the service and their capacity to provide the services, how supervision is done, how data on cervical cancer flow from the facilities to the district then to the National Cancer Registry, and how referral is managed within the district.
We selected women of European ancestry diagnosed with histologically verified primary invasive but not metastatic breast cancer (stage I to III disease) and restricted to women with available age information, because age is an important risk factor for breast cancer (flow chart of patient selection in Additional file 1: Figure S1).
Although common colon cancer lymphatic flow travels through the thoracic duct, Kawamura et al. demonstrated that the superficial abdominal lymphatic flow is the more important pathway in colon cancer with abdominal wall invasion, as that could lead to development of solitary ALNM [7].
In order to determine whether increased level of miR-875 or miR-3144 induce apoptosis of cervical cancer cell, flow cytometric analysis of cell apoptosis was performed for SiHa cells transfected with miR-875 or miR-3144 expression vectors, SiHa cells transfected with empty vectors (Vector) and untreated SiHa cells (Mock).
To establish a correlation between phenotypic response and the binding affinity of the LBD and NRR antibodies to Notch1 expressed on the surface of cancer cells, flow cytometry was performed with LS-1034, BxPColo_205_205, and TALL-1 cells using saturating concentrations of the WC75 (NRR) and WC613 (LBD) antibodies.
To examine the apoptotic effect of zinc on human prostate cancer cells, flow cytometric analyses were performed.
To measure the percentage distribution of P. indica extract-induced apoptotic and necrotic HeLa cancer cells, flow cytometry was performed.
Other measures of proliferation used in breast cancer include flow cytometric assessment of S-phase, mitotic index (a component of pathologic grade) and thymidylate synthase.
In breast cancer, previous flow cytometry studies have correlated DNA content with tumour grade (Hitchcock et al, 1989), p53 mutation status (Eissa et al, 1997; Friedrich et al, 1997), disease recurrence/relapse (Ewers et al, 1984; Stal et al, 1994a), and survival (Shiao et al, 1997; Utada et al, 1998), suggesting a therapeutic and prognostic clinical relevance.
First, we identified HLA-restricted peptides within the IDO protein to which spontaneous T-cell reactivity were detected in patients suffering from unrelated tumor types, i.e., melanoma, renal cell carcinoma and breast cancer by flow cytometry using HLA/peptide tetramers as well as in ELISPOT assays after in vitro stimulation but also in direct ex vivo assays.
To further examine the effect of HBA on cell cycle in leukemia and breast cancer cells, flow cytometry analyses were performed, where results showed cells were arrested in the G0/G1 phase of the cell cycle followed by a significant increase of THP-1, HL-60 and MCF-7 cells in the sub-G0/G1 phase.
