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Furthermore, by rapid electronic exchange and comparison of PFGE profiles, the Canadian cluster of human cases could be classified as unrelated to this outbreak.
The viral strain used for the experimental infections has a similar DNA sequence with a Canadian cluster of strains known to induce high mortality rates [ 12, 13].
We plotted Canadian samples alongside French samples, and found that the tight Canadian cluster overlapped the French cluster; outlying samples, on the other hand, did not intersect the French cluster at all (Supplementary Figure 2A).
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It appears that Canadian samples cluster closely with French samples; we investigated this in more detail and found that Canadian samples fell into two groups: a tight cluster, which corresponded with the French samples, and a loose cluster, which did not lie close to French samples.
In his opening remarks, Mr Philip Jennings, noted Canada's foundational strength in vaccines R&D conveying that "the federal government supports the growth of the Canadian vaccines cluster of expertise and the adjuvant sub-sector through continued investment and policy development".
For example, the WI/1/66 lineage clustered Canadian and United States isolates mainly from turkeys, mallard ducks, and shorebirds, and formed an independent North American cluster (Figure S1A H).
The Canadian isolates in clusters 1 to 3 have been identified from multiple provinces, including British Columbia, Alberta, Manitoba, Ontario, and Québec, representing western and central regions of Canada.
Canadian cities, for instance, cluster near the southern border; people who go north do so only seasonally.
Canadian samples tend to cluster with North-Central European and Scandinavian populations.
Phylogenetic analysis using the whole-genome data showed that the Canadian emm59 GAS organisms clustered tightly in the epidemic complex.
Phylogenetic analysis showed that all of the genes of A/Canada/1158/2006 clustered with Canadian swine isolates from 2005 (9 ) (data not shown).
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