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In this way, our own community and those outside can examine both these primary documents and Professor Abelson's analysis, which he is now forming through a careful process that includes a review of this written material as well as extensive in-person interviews.
Using antibody staining and image processing methods, it is thus possible to collect thousands of points on a scatter plot of input (Bicoid concentration) vs. output (Hunchback protein concentration); since even in a single embryo there are many nuclei that have the same Bcd concentration, one can examine both the mean Hunchback (Hb) response and its variance.
Due to the longer-term stability of 3-D cultures, assays can examine both initial targets and more integrated responses requiring immune-cell activation, proliferation/mito-suppression, fat accumulation, and adaptation over weeks of exposure.
On the other hand, the SEM analysis procedures can examine both direct and indirect effects of variables, as well as explore the complex causal relationship involved in the process [ 3, 18].
CART is a nonparametric method that can examine both linear and nonlinear interactions simultaneously, and it was designed to handle missing data by identifying and using surrogate variables to minimize ascertainment bias, as is the case in data collected for routine clinical care (9, 15 – 15).
To test the role of these two putative phosphorylation sites in APC function in an in vivo context where we can examine both cells receiving and not receiving Wnt signals, we turned to Drosophila, where we can express mutant APC2 under control of the endogenous APC2 promoter in the complete absence of all endogenous APC function, using embryos maternally and zygotically APC2 APC1 double mutant.
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It can either examine both sides of the issue in a balanced way or argue persuasively on one side only.
This method investigates the effects of acute exposures and can also examine both multiple exposures and interactions between exposures.
An important advantage of this method is it can directly examine both the equilibrium processes and interaction rates that occur between a drug and the soluble form of a protein, thus avoiding any effects immobilization may have on such interactions.
Two recent studies are described to illustrate how this workflow can be adapted to examine both known and unknown cellular complexes.
High-resolution computed tomography (hrCT) and magnetic resonance imaging (MRI) microscopy can be used to examine both cortical and trabecular bone in vivo.
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