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In contrast to aptamer beacons isolated from previous selection schemes,[ 2, 9– 12] these assays were simplified, as our aptamer beacons are already capable of fluorescence-based detection and can be utilized to detect analyte without any additional modification.
Different assays can be utilized to detect screen results, including luciferase reporter assay, Tat-dependent reporter assay, p24 detection, as well as GFP detection.
Furthermore, we demonstrate the utility of these SiNP ODN conjugates by illustrating how these particles can be utilized to detect oncogenic microRNA-21 (miR-21) via a fluorescence ON strategy.
Outlier statistic test such as w test (Baarda, 1968) can be utilized to detect and exclude outliers.
In regards to the fact that the product of iLAMP is DNA, molecular nanoprobes can be utilized to detect it.
The experimental results demonstrated that a strain-based methodology can be utilized to detect crack propagation in this type of composite joints.
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In addition to utilizing SPARTA together with a sequence simulator for simulating data sets that resemble an input MSA (as shown in fig. 5), our method can also be utilized to detect indel dynamics parameters for specific genes or lineages.
Secondary structure conservation can also be utilized to detect homologs of some ncRNAs based on characteristic combinations of sequence and structure motifs using special software tools designed for this purpose.
Intriguingly, because formaldehyde efficiently produces protein:nucleic acid as well as protein:protein crosslinks (13), this method can, in principle, be utilized to detect proteins that bind DNA directly as well as those that bind DNA through other factors.
The achieved points are potential lesion voxels which are utilized to detect breast lesions.
Next, qPCR was utilized to detect the inflammatory reactions at the mRNA expression levels.
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