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To assess whether protective immunity against TAP-negative tumor cells can be generated by immunization with either TAP1-expressing CMT.TAP1/B7.1 cells or TAP-negative CMT.B7.1/p cells, C57BL/6 mice were immunized with different amounts of γ-irradiated cells followed by challenge with CMT.64 cells.
Second, tumour-specific T cell responses can be generated by immunization of patients with peptides derived from tumour antigens and infused in soluble form or loaded onto dendritic cells.
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They can be generated by a simple computer code.
Any additional energy needs can be generated by renewable sources.
Studying the magnitude and longevity of the neutralizing antibody response during natural infection will help establish correlates of protection to be generated by immunization.
Antibodies were generated by immunization with recombinantly produced affinity-tagged target proteins.
These were generated by immunization with a steroidal hapten that mimics multicyclization without the necessity for anti-Markovnikov additions or ring expansions.
Tumor-antigen specific CTLs were generated by immunization of C57BL/6 mice with γ-irradiated CMT.B7.1/p cells (5×106 cells/mouse).
The VSV-Np epitope specific T cells were generated by immunization of mice with γ-irradiated 39.5/Kb cells pulsed with VSV-Np52 59 VSV-Np52 59
The anti-HIC serum was generated by immunization of rabbits with the peptide SGAGEALAPGPVG, comprising the first 13 amino acids of HIC.
The mAb against mouse uPA, mU1, was generated by immunization of uPA-deficient mice with a recombinant pro-form of mouse uPA [27].
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