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UAS- G- CaMP was obtained from Richard Axel (Department of Biochemistry and Molecular Biophysics, Columbia University).
The same level of accumulated cAMP was obtained at 5, 15 and 30 min at 4°C, regardless of whether the treatment was performed with cells attached to a plate (Fig. 2a, open circles) or in suspension (Fig 2a, closed circles).
Similar(58)
β, γ, δ and H30 affinity for cAMP were obtained from experimental measurements and were adjusted slightly to yield better agreement with experimental calibration data (Fig. 2).
Information on the relocation of households to IDP camps was obtained from the United Nations High Commission for Refugees.
N6-Bnz-cAMP and 8-pCPT-2'-O-Me-cAMP were obtained from Axxora LLC (San Diego CA).
H-CGP 12177, H-adenine, and C-cAMP were obtained from Amersham International (Little Chalfont, UK).
80, 81 Population data for refugee camps were obtained from recent estimates of the United Nations High Commission for Refugees (UNHCR) 82 and are not necessarily specific to the year 2010.
Human TIP39 failed to induce accumulation of cAMP with PTH1R and only negligible cAMP production was obtained for PTH3R (Additional file 4).
A purified CAMP factor was obtained by using a TALON resin column (Figure 1A, lane 3) and sequenced by a NanoLC-LTQ MS/MS mass spectrometer after in-gel trypsin digestion (Figure 1B).
Permission of the State Director of the NYSC and the state Camp Director was obtained before the commencement of the study.
cAMP assay kit was obtained from R&D (Minneapolis, MN, USA).
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