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Or could it be the result of having regular 6-minute pulses when cAMP is added exogenously, while on filters the signal might be more irregular?
When cAMP is added to cells it causes a cringe response, very similar to that induced by DIF-1 but with a transient increase in F-actin associated with the detergent-insoluble actin cytoskeleton rather than the decrease observed with DIF-1.
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In these experiments, 500 μM IBMX and 5 mM cAMP were added together into cultures, so that the activities were stabilized.
In 1912, the Bowen Country Club summer camp was added to complete the Hull House complex.
cAMP was added at the indicated concentrations.
Then cAMP was added for 5 h in a pulsatile fashion every 6 min to a final concentration of 30 nM.
Then, another 200 µl dose of 200 mM cAMP was added with 0.01 100 nM DIF.
Prior to initiation of the assay, cAMP was added to some samples to a final concentration of 30 μM.
The cultures were then divided, and the missing nutrient plus cAMP was added to one aliquot, while cAMP alone was added to the other, and then the samples were prepared for microarray analysis.
Renal explants derived from Pkd1 mutant mice do not develop cysts ex vivo unless analogues of cAMP are added to the culture medium [14].
Rather than developing on solid support, cells were starved in shaking suspension, and cAMP was added exogenously at concentrations and with a periodicity meant to mimic natural pulsatile signaling [ 13- 15].
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