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Intracellular cAMP extraction was followed as previously described [53].
The method for cAMP extraction using TCA was modified from a protocol for ATP extraction (Gustafsson, 1979).
Cells were harvested, washed and lysed with cAMP extraction buffer and cAMP levels were detected with the colourimetric cAMP Biotrak enzyme immunoassay according to the manufacturer's instructions (EIA, Amersham, Freiburg, Germany).
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We have earlier reported a lower percentage (44.7%) of satisfaction rates for visual acuity of 6/18 of better with the use of standard aphakic spectacles in a socio-epidemiological assessment after camp-based intracapsular cataract extraction in villages and peri-urban areas in north India [ 9].
When visiting scientists entrenched in the frigid business of unlocking the secrets of halogen particles on the Greenland ice sheet, she camped near the hole where extraction of two miles of ice cores in 1993 awakened the world to the swift pace of global warming.
The protesters have called their Somerset headquarters Camp Badger, potentially competing for headlines with environmental activists in other parts of southern England who have set up camp to frustrate plans for shale gas extraction.
Cell pellets were collected and extraction was prepared by using Amersham cAMP Biotrak EIA system (GE Healthcare, Little Chalfont, UK).
The metalworker Ruth Asawa and the weaver Kay Sekimachi were among the Californians of Japanese extraction who were sent to internment camps after Pearl Harbor.
Intracapsular cataract extraction has been the commonest mode adopted in eye camps in India.
Hundreds of protesters attended a six-day long Reclaim The Power camp in August near the Little Plumpton proposed site to campaign against shale gas extraction in the region by the controversial process of hydraulic fracturing, or fracking.
These patients had undergone intracapsular cataract extraction four years prior to the study either at an eye camp or at a base hospital in the rural areas and urban slums of north India and were selected randomly.
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