After incubation, cells were carefully rinsed with PBS and observed with an Axiovert 35 inverted fluorescence microscope equipped with a mercury-arc lamp and a CCD camera, driven by AxioVision software package (Carl Zeiss, Germany).
Digitized images of diaphragm sections examined were acquired using an upright microscope with camera driven by diagnostic software.
Thirty-one images per cell were acquired at 4-second intervals (see Video S1 and Video S2) using a digital camera driven by Metamorph software (Universal Imaging Corporation, Downingtown, PA) as previously described [22].
Live imaging was carried out using a spinning disc coupled to an Olympus BX-41 microscope (Ropert Scientific, France) (20×, NA 0,5 or 40×, NA 0,75 objective, CoolSnapHQ2 camera) driven by Metamorph software (Universal Imaging).
After the preparation was dried at room temperature, the neurons were investigated using a Zeiss Axiovert inverted microscope (Carl Zeiss, Oberkochen, Germany) coupled with a CCD camera, driven by the MCID software package (Imaging Research, Ontario, Canada).
Immunostaining was analyzed using as motorized Zeiss Axioplan 2 microscope equipped with a Hamamatsu C4742-95 digital camera driven by MetaMorph® 5.4 software.
The cultures were photographed at a Zeiss AxioStar Plus microscope equipped with a digital camera driven by AxioVision 4.6 software (Carl Zeiss, Germany).
Images were captured using a Philips CM10 transmission electron microscope at 80 kV equipped with a Morada 11 MPixel TEM CCD camera driven by the iTEM software (Olympus Soft Imaging Solutions).
Sections were viewed and photographed using a Zeiss LSM-510META scanning confocal microscope (Thornwood, NY) using a Zeiss axiocam HRm high-resolution CCD camera, driven by Axiovision 4.6 software.
The bryozoa and some loriciferans were also imaged using a Nikon Eclips 90i microscope fitted with DIC optics and a DS-5 M Nikon digital camera driven by Nikon ACT-2U software v.1.4.
Cells were treated with different drug solutions 20 min after seeding on Matrigel, and photographs were taken after 8-h-drug incubation using the × 5 objective of an Axiovert 200 M fluorescent microscope coupled to an AxioCamMR3 camera driven by the AxioVision 4.7 software (Carl Zeiss, North Ryde, New South Wales, Australia).
