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The in vivo Imaging System (IVIS), consists of a cooled camera (CCD) mounted on a light- tight chamber (dark box), a camera controller, a camera cooling system, and a Windows based computer system, were used for data acquisition and analysis.
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Each 96/24-well 96/24-wellle was plated in the sampleen chamber mounted wash the CCD camera cooled to −90°C, with a field of view set at 25 cm above the samplacedelf.
The two output channels were captured using an EMCCD camera cooled to −70 °C (Photometrics).
Images were acquired using a Hamamatsu ORCA II BT-512 camera cooled to -55°C in both horizontal and vertical arrangements.
An Andor iXon+ EMCCD camera cooled to −80 °C and Andor SOLIS software was used to record and process all videos and images.
The microscope was equipped with a CCD camera (cool SNAP HQ monochrome, Photometrics), and a beam-splitter optical device (Dual-channel simultaneous-imaging system, DV mag biosystem (ET-04-EM)).
The backscattered radiation was transmitted through a 50-μm diameter multimode fiber to a UHTS 300 spectrometer with a 600 groove mm 1 diffraction grating and back-illuminated CCD camera cooled to −65 °C (Newton DU970 N BV, Andor Technology Ltd., Belfast, UK).
The size of the beads was estimated from the photographic images acquired on a Zeiss Axiovert 25 CFL microscope (Zeiss, www.zeiss.de) using a SensiCAM camera (cooled CCD-chip, 640 × 480 pix, www.pco.de).
Bioluminescence was imaged using a 1.6 × Plan Apochromat objective (NA 0.24; Olympus) with 6.3 × zoom and transmitted to an electron-multiplying charge-coupled device (EM-CCD) camera cooled to −80 °C (ImagEM; Hamamatsu Photonics).
First they decided to blast a hole in the wall to install a huge air conditioning unit to keep the cameras cool (these are concrete buildings and it was not a small deal).
Images were captured using a Zeiss Microscope (Axiophot) equipped with a cooled-charge-coupled device (CCD) camera (Cool SNAP HQ, Photometric) and analyzed by IP lab software (Signal Analytics).
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