Note that, due to limited sensitivity and speed of camera acquisition, the technique is not fast enough to detect movements of cytosolic paxillin, which are on the submillisecond timescale.
The acquisition speed of the camera was up to 70,000 A-lines/s, and the interference spectrum data were transferred using the image acquisition board PCIe-14299, National Instruments, USA).
The maximum acquisition speed of the camera is 67k A-lines per second but hard drive writing limits acquisition to 15k A-lines per sec.
The central unit for achieving high acquisition speed is the CMOS camera in the spectrometer (Basler Sprint spL4096-140km).
If the images acquired from the camera are processed individually, there will be drawbacks such as slow image acquisition speed and loss of image information.
High speed CCD camera or swept source enables OCT or ODT to have higher signal acquisition speed, or higher temporal resolution of blood flow imaging system which allows for better reconstruction of the time course of dynamic processes.
Since high quantum efficiency line scan cameras are available with line rates up to 140 kHz, enhanced sensitivity and/or acquisition speed are realizable for sdLCS.
Imaging with TIRF microscopy on the previously described Nikon instrument was performed at 100% 488 nm laser power, 10 Hz acquisition speed, and with 2 × 2 binning of the pixels using the QuantEM camera.
For approaches that require mechanical switching of filters, multispectral acquisition speed is hindered by both the time required for switching and the need to trigger camera image acquisition in synchrony with wavelength switching rather than letting the camera free-run at its maximum speed.
An indium gallium arsenide (InGaAs) camera (SU1024, Goodrich Sensors Unlimited Inc, Princeton, NJ) was used, and the system could image at 7 frames per second with an acquisition speed of 7,000 A-lines/s, corresponding to a frame of 1,000 A-lines.
