The majority of the new Conserved Avian Microsatellite (CAM) markers amplified in all 12 species tested (on average, 94% in passerines and 95% in non-passerines).
This suggests that primer sequence similarity is the main factor affecting the identification of a polymorphic locus in this set of 24 CAM markers.
More than 90% of the remaining adherent cells had epithelioid morphology (Fig. 4A) and were positive for epithelial cell Ep-CAM marker as determined by scanning cytometry (Fig. 4B).
To identify the cell types that expressed L1-CAM-ir ring structures, we performed double immunostaining of L1-CAM with marker proteins such as glial fibrillary acidic protein (satellite cells) and ATF3 (injured neurons) (Tsujino et al., 2000).
In Ovarian cancer, M-CAM is a marker of early relapse and poorer outcome in EOC.
In NT, galanin neurone-specific expression was demonstrated by in situ hybridization counterstaining and immunohistochemical co-localization with N-Cam, a neuronal marker.
Double labeling with L1-CAM and ATF3, a marker of injured neurons, showed that most L1-CAM-ir ring structures were located in ATF3-labeled L5 DRG neurons in the nuclei at 7 days after SCNT (Fig. 3E).
In immunohistochemical staining, positive staining of α1-antichymotrypsin, α1-antitrypsin (pancreatic enzyme marker), AE1/AE3, CAM-5.2 and CK7 (epithelial marker), and MIB-1 index was <1 %.
Using global tests, five populations (AB, AR, AUX, CAM, PRE) and two markers (HMS3 and HTG10) showed significant deficit in heterozygotes (P < 0.01).
After 24 days, cells were stained with myogenic (myogenin, N-CAM) and neuronal markers (GFAP, N-CAM) (Figure 2C, D).
