Sentence examples for cam affinity from inspiring English sources

Protein was then purified by nickel immobilized metal affinity chromatography using Ni Cam affinity resin following the manufacturer's instructions Sigma-Aldrich (Steinheim, Germany).

Substitution of the chlorine atom with more polarizable halogens enhances both CaM affinity and biochemical activity (41), indicating that the bound state is hydrophobically stabilized.

Instead, regulation is achieved by cell surface modulation events that alter N-CAM affinity, prevalence, mobility, and distribution on the surface.

All three recombinant proteins were purified by CaM-affinity chromatography and steps involving thrombin digestion and concentration were omitted.

MdGAD3 is only the second reported plant GAD that cannot be purified by CaM-affinity chromatography and whose activity and spectral properties are not affected by Ca2+/CaM.

In a follow-up experiment, H MdGAD3 was compared to H AtGAD1, H AtGAD1ΔC32 and AtGAD1, with the first three recombinant proteins being purified by Ni2+-affinity chromatography and the fourth by CaM-affinity chromatography.

Because AtGAD1ΔC32, MdGAD3 and MdGADΔC34 could not be purified by CaM-affinity chromatography, a 6-His motif was added (designated by the prefix H on the protein name), prompting us to also investigate the impact of the tag on GAD activity.

In light of the previous experience with anti-Ep-CAM antibodies, we selected a moderate Ep-CAM binding affinity for development of MT201.

The results of the peptide screen were also inconclusive about the CaM-binding affinity of BD-C1.

This work demonstrates the use of the protein calmodulin, CaM, as an affinity tag for the reversible immobilization of enzymes on surfaces.

As calcineurin has such a high affinity for CaM, the effect of CaM-peptide on the interactions of CaM and other CaM-binding proteins should be examined.