Suggestions(1)
Exact(34)
This measure pSNP quantifies base calling errors and SNP calling errors [49].
Call clustering was inspected manually and adjusted when obvious calling errors were made by the software.
Besides the above procedure of selecting informative SNPs, we also suggest that users select SNPs to avoid mapping errors, SNP calling errors, as well as structural rearrangements.
Resulting chromatograms were manually trimmed and checked for calling errors.
However, our observation is unlikely to be a consequence of SNP calling errors for two reasons.
Other efforts have been made to understand the sources of variant calling errors [ 12].
Similar(26)
The substantial improvement in ortholog consistency arising simply from use of a different gene-calling algorithm illustrates the imperfection of gene calling and motivates the notion that inconsistent gene starts among closely related orthologs may often represent gene-calling errors.
However, there has been little work done in correcting base-calling errors in Ion Torrent data.
Potential gene-calling errors were identified by inconsistency in gene start positions among orthologs.
Inconsistencies will, of course, represent a mixture of true biological variation and gene-calling errors.
However, most of these inconsistencies appear to be gene-calling errors.
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